Jurnal Biosilampari : Jurnal Biologi

KAPASITAS POTENSI PARASITISME Trichogramma sp. (Hymenoptera: Trichogrammatidae) TERHADAP Corcyra sp.

Astrid Sri Wahyuni Sumah — 2023-05-03

Parasitism research Trichogramma sp. (Hymenoptera: Trichogrammatidae) has been carried out with the aim of studying the functional response of the parasitoid Trichogramma sp. Functional response is one measure to determine the effectiveness of a predator or parasitoid as an agent in biological control, so that it can express a change in the number of preys attacked by individual predators. Temperature is one of the most important host-parasitoid interactions in functional responses. The method used in the parasitoid parasitism Trichogramma sp. on the host Corcyra sp. using four levels of egg density Corcyra sp. with two different temperature treatments (27^OC and room temperature). The results show the functional response model of the parasitoid Trichogramma sp. against its host Corcyra sp. described in the type I model. Parasitization level of Trichogramma sp. showed the same results for both temperature treatments, however, it also shows differences in the speed of predation. It also showed linearity between the parasitoids Trichogramma sp. with the host Corcyra sp. Parasitism rates are still increasing, along with an increase in host density. This research can be a starting point to improve the control of Corcyra sp. using the parasitoid Trichogramma sp. in the field.

KEANEKARAGAMAN KELAS BIVALVIA DI PANTAI CIBUAYA UJUNG GENTENG

Anissyah Chaerul Putri — 2023-05-03

Cibuaya Beach is one of the beaches in Ujung Genteng Village that is used as a tourist destination because the beach is still very clean and natural. Cibuaya Beach has large beach sand grains and is dominated by mollusk shell fragments. The intertidal zone is the zone bounded by the tidal line and is the narrowest of the marine zones. The coastal ecosystem of Cibuaya Beach consists of coral reefs, seagrasses, and coastal vegetation. This study aims to determine the diversity of Bivalves in Cibuaya Beach Ujung Genteng. This research was conducted at Cibuaya Beach Ujung Genteng in March-April 2022. Determination of the research location using the belt transect method by purposive sampling at three stations namely rocky sand (station 1), seagrass (station 2), and coral reef (station 3). The results obtained were 148 individuals with 11 Bivalve species comprising six families. The value of the diversity index (H') ranged from 1.58-1.99 classified as medium. The value of the evenness index (E) ranges from 0.83-0.88 classified as high. The dominance index value (D) ranges from 0.18-0.24 which is low. The measurement results of abiotic parameters, namely temperature, pH, salinity, humidity, current speed, and light intensity show good values for marine bivalve life. Therefore, the Cibuaya Beach intertidal ecosystem is suitable for the life of the Bivalve class.

ISOLASI, ENUMERASI, DAN KARAKTERISASI BAKTERI PELARUT FOSFAT (BPF) INDIGENUS TANAH DESA KEBUNAN SUMENEP SEBAGAI AGEN PUPUK HAYATI ORGANIK

H. Hefdiyah — 2023-05-03

Phosphate Solubilizing Bacteria (PSB) are known that have important role to improve plant phosphate uptake as biofertilizer. These Microorganism hold potential to promote organic agriculture as eco friendly and efficiency. This study aims to isolate, enumerate and characterize indigenous PSB from Kebunan Village soil as the organic biofertilizer agent. Isolation and enumeration were carried out using the spread plate method on PCA media for enumeration of the total bacteria and Pikovskaya media (PKV) and NBRIP for BPF selection and enumeration. characterization includes observing the morphological colonies and cells. These experimental studies found that the soil pH was 6.5, the temperature range was 28-35 °C, the total P content was from 0.59 to 1.09 and the dissolved phosphate content sequentially from the smallest is 0.53 (KB2), 29.41 (KB1), 60.45 (KB3). The total abundance of bacteria at 3 different locations showed variations between 1.97 x 10⁶ to 287 x 10⁶. The highest abundance of BPF reached 5.45 x 10⁶ cfu/g was in the watershed which had the lowest available P content. Total 7 isolates of BPF were successfully isolated and characterized with different colony characters and cell shapes. The selected BPF isolates were dominated by Gram-positive bacilli.

STRUKTUR KOMUNITAS DIPLOPODA TANAH PADA KETINGGIAN BERBEDA DI PERKEBUNAN TEH KABAWETAN BENGKULU

Darmi Darmi — 2023-05-05

Soil diplopods are an important biotic component in agricultural land ecosystems. Its presence in the soil is an important in the decomposition process of soil organic matter, and plays a role in increasing soil fertility. This study aims to study the community structure of soil Diplopoda at different altitudes in the Bengkulu Kabawetan Tea Plantation. The location of the study used the stratified sampling method which is based on the altitude of 800 m, 900 m and 1000 m above sea level. Soil Diplopods were collected by using the 50x50 cm quadratic method and a 100 m line transect. Data analysis consisted of density, relative density, diversity index, and Morisita index. There were 4 types of soil Diplopods in the Bengkulu Kabawetan Tea Plantation, namely Arthrosphaera sp., Gyrodrepanum sp., Oxidus sp., and Trigoniulus sp. At an altitude of 900 m and 1000 m, 4 species of Diplopoda were found and at an altitude of 800 m consisted of 3 types of Diplopoda. The density of soil diplopods at the three study locations varied, with the highest density at an altitude of 1000 m followed by at an altitude of 800 m and at 900m. The diversity index at the three locations is low and moderate. The distribution of diplopods at the study site was random and uniform.

KEANEKARAGAMAN DAN POTENSI JENIS IKAN HASIL TANGKAPAN NELAYAN DI TEMPAT PELELANGAN IKAN (TPI) PAMAYANGSARI KABUPATEN TASIKMALAYA

Malina Mega Nuralam — 2023-05-05

This study aims to determine the diversity and potential of fish caught by fishermen at the Pamayangsari fish auction, Tasikmalaya Regency. This study uses descriptive quantitative methods with survey techniques. Data collection was carried out using catch data from January 2020 to October 2022 and conditional direct observations for 2 weeks in October 2022 carried out at the fish auction at Pamayangsari. The results showed that 58 fish species were identified at the fish auction of Pamayangsari, Tasikmalaya Regency, belonging to 2 classes, namely 55 species from the Actinopterygii class and 3 species from the Elasmobranchii class. As for the status of species diversity (H’) in the high category, the type wealth index (R1) is moderate, the evenness index (E) is high and stable, and the dominance index (C) is low which indicates that the community structure is quite stable. Overall catch results from January 2020 to October 2022. Based on these data the fish caught by fishermen landed at the fish auction of Pamayangsari, Tasikmalaya Regency, were quite abundant.

IDENTIFIKASI JENIS LEBAH MADU ASAL KEPULAUAN SULA MENGGUNAKAN APLIKASI DNA BARCODE LCO GEN

Abdu Mas'ud — 2023-05-16

Sanana Honey is a local brand, a type of honey produced by forest bees which is well known in the Sula Islands, North Maluku. The forest bees of the Sula Islands have the potential to be developed as a producer of quality honey. At this time there has been no scientific report regarding the types and characteristics of the forest bee species from the Sula Islands. This study aims to identify the types of honey-producing forest bees by applying the LCO gene DNA barcode. The methods used in this study used: 1) DNA extraction from bee body tissues using the DNA Miniprep Kit procedure (Zymo Research, D6016); 2). DNA amplification by PCR amplification procedure with TOYOBO KOD FX Neo (KFX-201); 3) bidirectional DNA sequencing process (Bi-directional sequencing). The results showed that the specimen was successfully amplified with an amplicon size of 677 bp. Furthermore, the results of BLASTN analysis revealed that the sequence has a 97.78% similarity with the Apis cerana mitochondria complete genome gene country of Russia, but is phylogenetically different between the two.

KEANEKARAGAMAN TUMBUHAN ANGIOSPERMAE DI KAWASAN TAMAN NASIONAL MATALAWA KABUPATEN SUMBA TIMUR

Wilfridus Balla Jawaray — 2023-06-01

The highest plant diversity in the tropical rainforest area in Matalawa National Park is the Angiospermae plant. There are still many people in Praingkareha Village who are not familiar with Angiospermae plants or closed seed plants. This study aims to describe the diversity index of Angiosperm plants found in the Laputi Forest block, Matalawa National Park. This research was limited to the Laputi Forest block, so it did not cover the entire forest in Praingkareha Village, Matalawa National Park, East Sumba Regency. This study used a double plot method with a purposive sampling technique. The results showed that there were 30 species of Angiosperms from 17 families with a total of 499 individuals. The diversity of Angiosperm plants in the Laputi Forest block of the Matalawa National Park area is moderate with (Ĥ)=2.90.

INVENTORY OF WEST JAVA BLACK LANGUR [Trachypithecus mauritius] FEEDING PLANTS IN THE BOGOR TAMAN SAFARI INDONESIAN FOREST AREA

Jansen Manansang — 2023-06-07

The West Java black langur is one of the endemic primates on the island of West Java. Currently, the conservation status of the West Java black langur (Trachypithecus mauritius) is categorized as Vulnerable status, registered in Appendix II. This research was carried out from January to March 2022. The purpose of the study was to determine the types of forage plants, and the parts eaten by the West Java black langur responsive found in the Taman Safari Indonesia (TSI) Bogor forest area. The research method used is the observation method through direct observation in the field. The results showed that there were 14 types of feed for the answering West Java black langur, divided into 12 families. The most dominant parts of the plant eaten by responsive West Java black langurs are young leaves 42%, shoots 30%, and others 28%.

BIOSINTESIS NANOPARTIKEL PERAK (AgNPs) DENGAN EKSTRAK DAUN KIRINYUH (Chromolaena odorata) TERIMPREGNASI ZEOLIT DALAM MENGHAMBAT BAKTERI PENYEBAB JERAWAT

Hulya Mariani — 2023-06-19

Acne is a chronic inflammation of the human skin. One of them is caused by the activity of the oil glands that produce excess sebum to cover the pores and is exacerbated by the presence of bacterial infections, namely Staphylococcus epidermidis and Propionibacterium acnes. Silver nanoparticles (AgNPs) are one of the most widely synthesized uses of nanotechnology because they are toxic to bacterial cells so that they can be used as antibacterial agents. This study aimed for the biosynthesis effectivity against acnes bacteria. The method was using experimental research with analyze the data through ANOVA. The results showed that zeolite-impregnated AgNPs had the potential as antibacterial agents against acne-causing bacteria that had strong inhibition as evidenced by the diameter of the inhibition zone formed on Staphylococcus epidermidis and Propionibacterium acnes in the range of 11-20 mm at any given concentration used. Based on the average diameter of the inhibition zone formed, it can be seen that the largest inhibitory zone occurred at 15 mm and 20 mm AgNO₃, with 18 mm in Staphylococcus epidermidis and 14.6 mm in Propionibacterium acnes bacteria respectively. This study was the first initially test the AgNPs effect to the acne’s bacteria and its further potential for anti-acnes.

PENGARUH KONSUMSI MINUMAN SEGAR BUAH Etlingera hemisphaerica (MSBE) TERHADAP KADAR GLUKOSA DARAH PADA PEKERJA TAMBANG EMAS

Fenty Fitrian Sari — 2023-06-27

Etlingera hemisphaerica has been used by the community for generations as medicine. E. hemisphaerica contains flavonoids, saponins, tannins which can lower blood glucose levels. This study aims to determine the effect of giving E. hemisphaerica fruit fresh drink (MSBE) on blood glucose levels. This research is a limited clinical trial with trials of measuring blood glucose levels before and after treatment. The research prospectus consisted of gold mine workers and non-workers. Data collection was carried out before and after giving MSBE for 7 days to the probandus. Based on the T test, the results obtained are t hit ˃ t tab. In 10 probandus, the average blood glucose level before treatment was 117.5 mg/dL and blood glucose level after treatment was 93.4 mg/dL. Whereas in the 5 probands of mining workers, the average blood glucose level before treatment was 133,200 mg/dL and blood glucose level after treatment was 100,400 mg/dL. In the 5 non-mining probandus, the average blood glucose level before treatment was 101,800 mg/dL and blood glucose level after treatment was 86,400 mg/dL. Based on the results of the study it was concluded that MSBE administration had the potential to reduce blood glucose levels in 10 probandus.

ANALISIS ANGKA LEMPENG TOTAL BAKTERI PETIS KUPANG PUTIH (Corbula faba) INDUSTRI RUMAH TANGGA DI DESA SUNGIKULON KECAMATAN POHJENTREK KABUPATEN PASURUAN

Senja Ikerismawati — 2023-06-27

White mussel (Corbula faba) is one of the fishery products belonging to the shellfish group. White mussel can be used as processed food. White mussel petis is a processed food that comes from the utilization of leftover boiled white clams mixed with granulated sugar and brown sugar and then boiled until thickened. This study aimed to analyze total plate count bacteria in the home industry for making white mussel paste in Sungikulon village, Pohjentrek, Pasuruan. This study used the total plate count method pouring technique. Analysis total plate count carried out at the Surabaya Health Laboratory Center, by testing six samples of white mussel petis and three repetitions, test data total plate count white mussel petis will be compared with SNI 2718.1:2013 to determine the quality of the sample.The results of this study indicate that the two samples do not meet the SNI 2718.1: 2013 standard, namely the SK1 sample is 82 x 10³ and the SK6 sample is 22.9 x 10^3.. There are four samples whose values are lower than SNI 2718.1: 2013, namely the SK4 sample of 0.01 x 10^3. SK2 sample of 0.05 x 10³. Sample SK3 is 0,11 x 10³ and SK5 sample is 0.42 x 10³. This means that the quality of the four samples is good.

AKTIVITAS ANTIJAMUR PELAPUK KAYU NANOPARTIKEL KITOSAN-EKSTRAK N-HEKSAN DAGING BUAH KAYU GADIS (Cinnamomum parthenoxylon)

Munifilia Ekasari — 2023-06-30

This study aims to synthesize chitosan nanoparticles - n-hexane extract of Cinnamomum parthenoxylon (CP) fruit using the ionic gelation method, to characterize them, and to determine their antifungal activity against the wood-rotting fungi Trametes versicolor and Fomitopsis palustris. Chitosan-CP extract nanoparticles were formulated with chitosan:Na-TPP:CP extract ratio of 1:1:0.5. Particle size and polydispersity index were determined using a particle size analyzer (PSA), and particles morphology was observed using a scanning electron microscope (SEM), and antifungal activity was measured using agar media. The results revealed that the average size of chitosan nanoparticles (base) was 276.9 nm, while the average size of chitosan-CP extract nanoparticles was 807.6 nm. The polydispersity index (PI) of both nanoparticles was greater than 0.7. Observations of the nanoparticles' morphology revealed that aggregation/agglomeration formed in the resulting particles. Both nanoparticles chitosan (base) and chitosan-CP extract inhibited the growth of white rot fungi (T. versicolor) more than brown rot fungi (F. palustris), as measured by the inhibitory zone.

POLA PERTUMBUHAN DAN FAKTOR KONDISI IKAN LELE PANJANG (Clarias leiacanthus ) DI AEK SILOM-LOM, LABUHANBATU

Aweng Awendi — 2023-07-08

This study was aimed at analyzing the growth patterns of catfish (Clarias leiacanthus) through the analysis of length weight relationships. The Condition Factor is calculated to determine the habitat conditions of the C. leiacanthus fish population. The research was carried out from October to December 2021 in the Aek Silom-lom River, South Labuhanbatu Regency. Measurement of fish length and weight and identification were carried out at the Ecology Laboratory, Faculty of Teacher Training and Education, Labuhanbatu University. Samples were captured using fishing gear in the form of fishing rods. Growth pattern analysis refers to the Linear Allometric Model (MAL), the condition factor is calculated based on relative weight (Wr) and Fulton (K). The catch during the study was 54 catfish (C. leiacanthus) obtained from Aek Silom-lom. The results of measurements against the sample obtained that the total length range (TL) is 20-31 cm, and weight (W) 81-180 grams. Analysis of the linear allometric model explained that the growth pattern of catfish (C. leiacanthus) is negative allometric b=2.22 (b<3). The results of the condition factor analysis obtained a relative range (Wr) of 81.047 – 124.552 with an average of 88.01. Factor Analysis of Fulton's condition (K) found a K value of 0.84. Based on the value of the Fulton condition factor (K) it can be concluded that the waters of Aek Silom-lom are in a balanced state, and can support the life of long catfish (C. leiacanthus).

Cover Biosilampari 5(2)

Editor Editor — 2023-07-04