BIOREMEDIASI Pb OLEH BAKTERI INDIGEN LIMBAH CAIR AGAR

Senja Ikerismawati

doi:10.31540/biosilampari.v1i2.288

Senja Ikerismawati Universitas Yudharta Pasuruan

DOI: https://doi.org/10.31540/biosilampari.v1i2.288

Keywords: Bioremediation, Indigenous, Bacteria, Pb, Agar Liquid Waste

Abstract

Bacteria isolated from ad environment contaminated with heavy metals are very potential as heavy metal bioremediation agents called indigenous bacteria. The purpose of this study was to isolate and identify indigenic bacteria that have the potential as Pb bioremediation agents in agar liquid waste and to analyze the ability of indigenic bacteria in reducing Pb. The research design used Completely Randomized Design (RAL) two factorial with variation of bacteria and eight days of treatment. Data were analyzed using two-way ANOVA with Duncan's advanced test. The results showed that there were eight isolates resulting from the isolation of agar liquid waste. The isolates of liquid waste indigen bacteria so that the most potential in reducing Pb were isolated H, E and F. The three isolates were able to reduce Pb in sterile agar liquid waste by 82.6%, 81.3% and 79.3% for eight days of treatment. The identification results using Microbact TM GNB 12A / B / E, 24 Identification Kits showed that H bacterial isolates were Bacillus alvei, E isolates were Bacillus pumilus species and F isolates were Bacillus lichenformis species

References

Chojnacka, K. (2010). Biosorption and Bioaccumulation The Prospects for Practical Applications. Environment International, 39 (3): 299-307.
Fidiastuti, H., & Suarsini, E. (2017). Potensi Bakteri Indigen dalam Mendegradasi Limbah Cair Pabrik Kulit Secara In Vitro. Bioeksperimen: Jurnal Penelitian Biologi, 3(1), 1-10.
Gerdhart, K.E., Huang, X., Bernard R., Greenberg., and Bruce, M. (2008). Phytoremediation of organic soil contaminants: Potential and Challenges. Plant Science, 176 (1):20-30.
Gusnita, D. (2012). Pencemaran Logam Berat Timbal Di Udara dan Upaya Penghapusan Bensin Bertimbal. Berita Dirgantara, 13 (3): 95-101.
Gupta, V.K dan Rastolgi, A. (2008). Biosorption of Lead (II) From Aques Solutions by Non Living Algal Biomass Oedogonium sp. and Nostoc sp.: A Comparative Study, Colloids and Surfaces. Colloids and Surfaces B: Biointerfaces, 64 (2): 170-178.
Khasanah, Eliya. (2009). Adsorpsi Logam Berat. Oseana, XXXIV(4), 1-7.
Lutfi, SR., Wignyanto, & Kurniati, E. (2018). Bioremediasi Merkuri Menggunakan Bakteri Indigenus dari Limbah Penambangan Emas di Tumpang Pitu Banyuwangi. Jurnal Teknologi Pertanian, 19 (1): 15-24.
Naik, M.M, & Dubey, S.K. (2013). Lead Resistant Bacteria: Lead resistance mechanisms, their application in lead bioremediation and biomonitoring. Ecotoxicology and Environmental Safety, 98(1), 1-7.
Pambudiono, A., Suarsini, E., & Amin, M. (2017) The Potensial of Indigenous Bacteria for Removing Cadmium from Industrial Wastewater in Lawang East Java. The Jurnal of Tropical Life Science, 8(1), 62-67.
Park, J., Bolan, N., Megharaj, M & Naidu, R. (2010). Isolation of Phosphate-Solubilizing Bacteria and Characterization of Their Effect on Lead Immobilization. Pedologist, 53(3): 67-75
Peraturan Pemerintah Republik Indonesia Nomor 82 Tahun 2001. Tentang Pengelolaan Kualitas Air dan Pengendalian Pencemaran Air.
Pudji S.R, Sumingkrat, Noer. S.T, Agustina S, Trisni A, Rofienda, & Deni. (2006). Penelitian Bioremediasi (Ex-Situ) Tanah Terkontaminasi Limbah B3 Yang Mengandung Logam Berat. Buletin Penelitian, 28 (1): 8-17.
Suhendrayatna. (2001). Bioremoval Logam Berat dengan Menggunakan Microorganime: Suatu Kajian Kepustakaan (Heavy metal bioremoval by microorganisms : A literature study). Prosiding Bioteknologi untuk Indonesia Abad 21. Sinergy Forum - PPI Tokyo Institute of Technology

Suyasa, I.WB. (2012). Bakteri Pendegradasi Minyak Dari Beberapa Sedimen Perairan Tercemar dan Bak Penampungan Limbah. Bumi Lestari Journal of Environment, 7(1): 1-6.
Spain, A. (2003). Implications of microbial heavy metal tolerance in the environment. Reviews in Undergraduate Research 2, 1-6.
Vijayaraghavan, K. & Yun, Y.S. (2008). Bacterial biosorbents and biosorption. Biotechnology Advances., 26 (3): 266-291.
Zahoor, A. & Rehman, A. (2009). Isolation of Cr(VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater. Journal of Environmental Sciences , 21 (6): 814 – 820
Zulaika, E. (2012). Bakteri Resisten Logam Berat Yang Berpotensi Sebagai Biosorben dan Bioakumulator. Seminar Nasional Waste Managament For Suistainable Urban Developing Teknik Lingkungan ITS. Surabaya: ITS