Pilot Study: Pemanas Untuk Thermal Cycler
Abstract
During the Covid-19 pandemic, DNA amplification technique – the Polymerase Chain Reaction (PCR) method became the gold standard for virus diagnosis and measuring the amount of viral target nucleic acid in a clinical sample and delivering quantitative results. This PCR method is implemented as Thermal Cycler or PCR machine. One of the main components in the Thermal Cycler is the heater. Three heaters, such as: Peltier Device 12715, Ceramic Heater PTC 12 VDC, and Customized Heater 12 VDC were tested for their performance by being connected to 3-well aluminium block. Two pieces of PTC Ceramic Heaters 12 VDC with a total power of 120 W showed the best performance compared to the other 2 heaters and were able to provide a heating ramp rate of 0,13 /second.
References
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STMicroelectronics. (2017). VNH2SP30-E - Automotive fully integrated H-bridge motor driver. In VNH2SP30-E Datasheet (pp. 1–35). https://www.st.com/en/automotive-analog-and-power/vnh2sp30-e.html
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Wicaksono, N. B., & Effendi, S. M. (2022). Heating and Cooling Rate Study on Water Cooling Thermal Cycler using Aluminium Block Sample. Journal of Electronics, Electromedical Engineering, and Medical Informatics, 4(2), 55–61. https://doi.org/10.35882/jeeemi.v4i2.1
Wong, G., Wong, I., Chan, K., Hsieh, Y., & Wong, S. (2015). A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings. PLOS ONE, 10(7), e0131701-. https://doi.org/https://doi.org/10.1371/journal.pone.0131701
Lo, Y. M. D., & Chan, K. C. A. (2006). Introduction to the polymerase chain reaction. Methods in Molecular Biology (Clifton, N.J.), 336, 1—10. https://doi.org/10.1385/1-59745-074-x:1
Logan, J. M. J., & Edwards, K. J. (2009). An Overview of Real-Time PCR Platforms. In J. Logan, K. Edwards, & N. Saunders (Eds.), Real-time PCR: Current Technology and Applications. Caister Academic Press.
Maxim Integrated. (2015). Cold-Junction Compensated Thermocouple-to-Digital Converter. In MAX31855 Data sheet. Maxim Integrated.
Miralles, V., Huerre, A., Malloggi, F., & Jullien, M.-C. (2013). A Review of Heating and Temperature Control in Microfluidic Systems: Techniques and Applications. Diagnostics, 3(1), 33–67. https://doi.org/https://doi.org/10.3390/diagnostics3010033
Mullis, K. B., & Faloona, F. A. (1987). [21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. In Recombinant DNA Part F (Vol. 155, pp. 335–350). Academic Press. https://doi.org/https://doi.org/10.1016/0076-6879(87)55023-6
Regional Office for South-East Asia, W. H. O. (2016). Establishment of PCR laboratory in developing countries, 2nd edition. WHO Regional Office for South-East Asia. https://apps.who.int/iris/handle/10665/249549
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985). Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia. Science, 230(4732), 1350–1354. https://doi.org/10.1126/science.2999980
Sailaja, V., & Raju, K. N. (2019). A Review on Heating and Cooling system using Thermo electric Modules. IOSR Journal of Electrical and Electronics Engineering (IOSR-JEEE), 14(1), 49–57. https://doi.org/https://doi.org/10.9790/1676-1402014957
STMicroelectronics. (2017). VNH2SP30-E - Automotive fully integrated H-bridge motor driver. In VNH2SP30-E Datasheet (pp. 1–35). https://www.st.com/en/automotive-analog-and-power/vnh2sp30-e.html
Tahamtan, A., & Ardebili, A. (2020). Real-time RT-PCR in COVID-19 detection: issues affecting the results. Expert Review of Molecular Diagnostics, 20. https://doi.org/10.1080/14737159.2020.1757437
Tellurex Corporation. (2010). Frequently Asked Questions About Our Cooling And Heating Technology. www.tellurex.com
Wicaksono, N. B. (2013). Mesin PCR. In R. Mengko (Ed.), Instrumentasi Laboratorium Klinik (pp. 125–137). Penerbit ITB.
Wicaksono, N. B., & Effendi, S. M. (2022). Heating and Cooling Rate Study on Water Cooling Thermal Cycler using Aluminium Block Sample. Journal of Electronics, Electromedical Engineering, and Medical Informatics, 4(2), 55–61. https://doi.org/10.35882/jeeemi.v4i2.1
Wong, G., Wong, I., Chan, K., Hsieh, Y., & Wong, S. (2015). A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings. PLOS ONE, 10(7), e0131701-. https://doi.org/https://doi.org/10.1371/journal.pone.0131701
Published
2023-06-02
Section
Articles
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